Sens4All

 

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Enhancing Rapid Tests for Worldwide Diagnostics

 

BIO2016-75751-R  SENS4ALL. Enhancing Rapid Tests for Worldwide Diagnostics

Ministerio de Economía y Competitividad

SUMMARY

The aim of Sens4all is to contribute in the development of the next generation of rapid diagnostic test (RDTs) for low-resource settings to solve the societal challenge of Global Health. This proposal explore three key cross-cutting technological challenges that have been identified as technology bottlenecks in RDTs (i) the isolation of targets from complex specimens by novel solid-phase preconcentration strategies and advanced materials including magnetic molecularly-imprinted polymeric particles (ii) the enhancement of the analytical signal by isothermal amplification techniques of nucleic acid targets, and (iii) the study of emerging diagnostic biomarkers for the early detection of global diseases based on exosomes. Finally, the integration of these technological challenges in RDTs are explored. Hand-held electrochemical biosensors operated by batteries will be considered if quantitative results are required. Lateral flow tests with visual readout, as well as other non-conventional formats based on the same technology, will be designed whether yes/no qualitative information is needed. Analytical simplification are  implemented in all cases to provide low complexity and affordable platforms requiring minimal training for final users, but without any lost in the diagnostic accuracy. The application that are envisaged includes targets affecting global health, taking as a reference communicable diseases and emerging infectious diseases disregarding borders (such as Ebola virus), non-communicable diseases (for instance breast cancer and diabetes), and targets affecting water supplies (waterborne pathogenic bacteria and other pollutants). Although most of these major diseases are treatable, the early identification of individuals requiring treatment remains a major issue. The final goal of Sens4all is then reducing the burden of global diseases by improving the analytical performance of RDTs, as the sensitivity, specificity and analytical simplification. These RDTs hold great potential because they will be easily introduced into the general practitioner’s armory at the community and primary care level in low-resource settings.

KEY WORDS

bionanotechnology, biosensors, RDTs, magnetic particles, exosomes, isothermal amplification, global health