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Separation methods of biomolecules. Electrophoresis of proteins and DNA. Extra materials #2

Lesson 3

Separation methods of biomolecules. Electrophoresis of proteins and DNA. Gel Electrophoresis (GE). SDS Polyacrylamide Gel Electrophoresis (SDS–PAGE). Southern, Northern and Western blot. Applications of Electrophoresis. Diagnosis of Genetic (Inherited) Disease. Paternity and forensic testing. DNA Sequencing. Diagnosis of infection diseases. 

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This lecture will be focused on the separation of DNA and proteins by gel electrophoresis and all the applications involved.

Warning! typo in minute 3:46, instead of vertical is horizontal, since I am talking about DNA separation

Southern blot

PROBLEMS and EXERCISES

Separation methods of biomolecules. Part 1 Electrophoresis

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Podcast for virtual blackboard (for problems 1 to 4)

Podcast for virtual blackboard (for problems 5, 7, 8, 9, 10, 15)

Podcast for virtual blackboard (for problems 6, 11, 12, 13, 14)

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Podcast for virtual blackboard (for problems 18 and 19)

EXTRA CONTENT. MULTIMEDIA

It is highly recommended to watch the videos (add the subtitles if necessary). for further understanding of the technique

Remember that you can find more multimedia content in Selected videos and multimedia (password required)

Principles in electrophoresis

Gel electrophoresis

Electrophoresis SDS-PAGE

Restriction fragments Southern Blot (important)

Crossword

Instructions. Solve the crossword, be the first in doing a screenshot and post it in the forum under the topic Top five challenge!


1. The movement of electrically charged molecules under an electrical field
2. Reagent which is used for reducing disulphide bonds
3. Gel used for the detection of proteins
4. Crosslinker in PAGE
5. DNA moves towards the....
6. Gel used for the detection of DNA
7. Acronym for polyacrylamide gel electrophoresis
8. Blotting technique for DNA
9. Movement of liquid through a capillary tube or membrane under the influence of an electric field
10. Blotting technique for proteins
11. DNA is frequently cut into smaller fragments using a DNA ..... endonuclease enzyme
12. Blue dye used for coloring proteins
13. Blue dye used for tracking the gel electrophoresis
14. A protein with an isoelectric point of 9 will move at pH 7 towards the
15. Fragmentation pattern of DNA
16. Ion exchange chromatography separates and purifies analytes according to their overall
17. Chromatography based on an specific reaction
18. Common detection methods for liquid chromatography of biomolecules
19. In siseexclusion chromatography, the bigger molecule elute
20. Most popular and commonly used anion exchanger

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