ExoSensPoC

ExoSens-PoC. Enhancing tests for the early diagnosis of Alzheimer’s disease.

Alzheimer’s Disease (AD) is the major single cause of dementia. It courses with a long prodromal period, which difficult the identification of patients in early stages in which they might benefit from disease modifying treatments. Existing diagnostic tools such as brain imaging or cerebrospinal fluid biomarkers are expensive and invasive procedures, limiting their appeal to patients. Exosomes, small extracellular vesicles secreted by most cell types, have been recently proposed as carriers of biomarkers for many non-transmissible diseases as their cargo reflects cellular processes happening in the cell of origin. In the context of AD, they have been shown to be carriers of AD-related molecules. These vesicles can be found in many biofluids, such as plasma, therefore making them a suitable target for non-invasive biomarker research. ExoSens-PoC proposal addresses the development of a Proof-of-Concept/Point-of-Care (PoC) test for the early identification of individuals at risk of AD in plasma. Such a test can be used both in low resource settings where current technologies are not available, and in high-income countries at community and primary-care level to dramatically improve turnaround times for results and reduce costs. The device is rapid and inexpensive, requiring minimal handling, and can easily be introduced into the general practitioner’s armory for ambulatory screening of AD and risk-stratification of the patients, increasing thus its valorization.

Prof. Isabel Pividori, experts in the Diagnostic Tests based on exosomes will partner with ACE foundation/ Institut Català de Neurociències Aplicades and EureCat in this acceleration phase, to build a reliable, scalable, and secure product faster and at the same time, ensuring compliance with the EU Regulation.

This two year project is funded by the European Union NextGenerationEU.

Publications

2024

Peptide-based biosensing approaches for targeting breast cancer-derived exosomes. Rafael da Fonseca Alves, Arnau Pallarès-Rusiñol, Rosanna Rossi, Merce Martí , Emilia Rezende Vaz, Thaise Gonçalves de Araújo, Maria Del Pilar Taboada Sotomayor and Maria Isabel Pividori. Biosensors and Bioelectronics 255, 2024, 116211. https://doi.org/10.1016/j.bios.2024.116211

Los exosomas. Nuevas herramientas para el diagnóstico de enfermedades no transmisibles en biopsias líquidas. Rosanna Rossi, Arnau Pallarès-Rusiñol, Mercé Martí, María Isabel Pividori. Actualidad Analítica 85, 2024, 15-20. ISSN 2444-8818. Sociedad española de química analítica. 

2023

Magnetic Separation of Cell-Secreted Vesicles with Tailored Magnetic Particles and Downstream Applications. Bernuz, M., Pallarès-Rusiñol, A., Rossi, R., Fernández-Senac, C., Martí, M., Pividori, M.I. (2023). In: Vainio, S. (eds) Cell-Secreted Vesicles. ‘Methods in Molecular Biology’, vol 2668. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3203-1_18

Plasma extracellular vesicles reveal early molecular differences in amyloid positive patients with early-onset mild cognitive impairment. Amanda Cano, Pablo García, Mireia Bernuz, Raquel Puerta, Itziar de Rojas, Ester Esteban-De Antonio, Alba Pérez-Cordón, Laura Montreal, Raúl Núñez, Óscar Sotolongo-Grau, Silvia Alonso-Lana, Emilio Alarcón-Martín, Sergi Valero, Montserrat Alegret, Joan Martínez, Elvira Martín, Miren Ettcheto, Assumpta Vivas, Marta Gomez-Chiari, Miguel Ángel Tejero, Adelina Orellana, Lluís Tárraga, Marta Marquié, Mercè Martí, María Isabel Pividori, Mercè Boada, Agustín Ruiz, Journal of Nanobiotechnology 2023, 21 (1), 1-19. https://doi.org/10.1186/s12951-023-01793-7

Electrochemical genosensing of overexpressed GAPDH transcripts in breast cancer exosomes. A Pallarès Rusiñol, Lima de Moura, M Martí, MI Pividori. Electrochemical genosensing of overexpressed GAPDH transcripts in breast cancer exosomes. Analytical chemistry, 2023, 95, 2487–2495  https://doi.org/10.1021/acs.analchem.2c04773. 

Experimental data of the manuscript “Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer Exosomes”. A Pallarès Rusiñol, Lima de Moura, M Martí, MI Pividori. Electrochemical genosensing of overexpressed GAPDH transcripts in breast cancer exosomes. 2023 | Data set. DOI: 10.34810/DATA681. 

Advances in exosome analysis. A Pallares-Rusiñol, M Bernuz, S L Moura, C Fernández-Senac, R Rossi, M Martí and MI Pividori, in Advances in Clinical Chemistry 2023,112:69-117  https://doi.org/10.1016/bs.acc.2022.09.002

Extracellular vesicles, the emerging mirrors of brain physiopathology. Cano A, Ettcheto M, Bernuz M, Puerta R, Esteban de Antonio E, Sánchez-López E, Souto EB, Camins A, Martí M, Pividori MI, Boada M, Ruiz A. International Journal of Biological Sciences 19(3):721-743. doi:10.7150/ijbs.79063