ExoSenS

ExoSenS. The exosomes as diagnostic biomarkers in biosensors

Exosomes are cell‐derived nanovesicles released into biological fluids, which are involved in physiological cell-to-cell communication, to maintain natural homeostasis and pathological responses. These vesicles are under intensive study as biomarkers for improving the clinical diagnosis and prognosis of different conditions. This proposal explores the use of the exosomes as a target in rapid test (RDTs) for the early diagnosis of communicable and non-communicable diseases and to meet the features for RDTs. All the developed strategies will assess a coherent business-focused analysis of research and innovation bottlenecks and opportunities to further societal challenges related with health and wellbeing.

This three year project is funded by Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i y del Programa Estatal de I+D+i Orientada a los Retos de la Sociedad, en el marco del Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020.

Most important results

Publications

2023

Plasma extracellular vesicles reveal early molecular differences in amyloid positive patients with early-onset mild cognitive impairment. Amanda Cano, Pablo García, Mireia Bernuz, Raquel Puerta, Itziar de Rojas, Ester Esteban-De Antonio, Alba Pérez-Cordón, Laura Montreal, Raúl Núñez, Óscar Sotolongo-Grau, Silvia Alonso-Lana, Emilio Alarcón-Martín, Sergi Valero, Montserrat Alegret, Joan Martínez, Elvira Martín, Miren Ettcheto, Assumpta Vivas, Marta Gomez-Chiari, Miguel Ángel Tejero, Adelina Orellana, Lluís Tárraga, Marta Marquié, Mercè Martí, María Isabel Pividori, Mercè Boada, Agustín Ruiz, Journal of Nanobiotechnology 2023, 21 (1), 1-19. https://doi.org/10.1186/s12951-023-01793-7

Electrochemical genosensing of overexpressed GAPDH transcripts in breast cancer exosomes. A Pallarès Rusiñol, Lima de Moura, M Martí, MI Pividori. Electrochemical genosensing of overexpressed GAPDH transcripts in breast cancer exosomes. Analytical chemistry, 2023, 95, 2487–2495  https://doi.org/10.1021/acs.analchem.2c04773

Advances in exosome analysis. A Pallares-Rusiñol, M Bernuz, S L Moura, C Fernández-Senac, R Rossi, M Martí and MI Pividori, in Advances in Clinical Chemistry 2023,112:69-117  https://doi.org/10.1016/bs.acc.2022.09.002

Extracellular vesicles, the emerging mirrors of brain physiopathology. Cano A, Ettcheto M, Bernuz M, Puerta R, Esteban de Antonio E, Sánchez-López E, Souto EB, Camins A, Martí M, Pividori MI, Boada M, Ruiz A. International Journal of Biological Sciences 19(3):721-743. doi:10.7150/ijbs.79063 

Magnetic Separation of Cell-Secreted Vesicles with Tailored Magnetic Particles and Downstream Applications. Bernuz, M., Pallarès-Rusiñol, A., Rossi, R., Fernández-Senac, C., Martí, M., Pividori, M.I. (2023). In: Vainio, S. (eds) Cell-Secreted Vesicles. ‘Methods in Molecular Biology’, vol 2668. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3203-1_18

2022

The activity of alkaline phosphatase in breast cancer exosomes simplifies the biosensing design. Silio Lima de Moura, Arnau Pallarès Rusiñol, Luciano Sappia, Mercè Martí and MI Pividori. Biosensors and Bioelectronics 198, 113826. https://doi.org/10.1016/j.bios.2021.113826

Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice. Tang X, Zhang Q, Pividori MI, Zhang Z, Marty J-L, Catanante G. A. Biosensors. 2022; 12(2):59. https://doi.org/10.3390/bios12020059

Electrochemical glyphosate sensor based on hybrid material SiO2/Sm2O3/carbon modified with Meldola Blue. R. Blasques, A. Mendes A. Costa, T. Canevari, L.Arenas; (…) MI Pividori, Paulo Villis, Electroanalysis 2022, 34, 1– 13. https://doi.org/10.1002/elan.202200373

2021

Immunomagnetic separation improves the detection of mycobacteria by paper-based lateral and vertical flow immunochromatographic assays. A Ben Aissa, B Araújo, E Julián, MV Boldrin Zanoni and MI Pividori. Sensors 2021, 21(18), 5992; https://doi.org/10.3390/s21185992. Selected for the cover.

Magnetic-molecularly imprinted polymers in electrochemical sensors and biosensors. J. Marfà, R.R. Pupin, MPT Sotomayor & MI. Pividori. Analytical and Bioanalytical Chemistry 2021; https://doi.org/10.1007/s00216-021-03461-x

Development of magnetic nanoparticles modified with new molecularly imprinted polymer (MIPs) for selective analysis of glutathione. AC Ferreira Santos, ORP de Araújo, FA Moura, S Khan, AA Tanaka, AEG Santana, MI Pividori, MDPT Sotomayor, MOF Goulart. Sensors and Actuators B: Chemical, 2021, 344, 130171; https://doi.org/10.1016/j.snb.2021.130171. 

Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay. Juan Carlos Porras, Mireia Bernuz, Jennifer Marfa, Arnau Pallares-Rusiñol, Mercè Martí and María Isabel Pividori, 2021, Nanomaterials 2021, 11(3), 741; https://doi.org/10.3390/nano11030741

Assessment of the biological potential of diaryltriazene-derived triazene compounds. Patricia de Maria Silva Figueirêdo, …, María Isabel Pividori, Manfredo Hörner & Paulo Cesar Mendes Villis. Scientific Reports volume 11, Article number: 2541 (2021). 

Electrochemical Genosensing of E. coli Based on Padlock Probes and Rolling Circle Amplification. Alejandra Ben Aissa, Narayanan Madaboosi, Mats Nilsson and Maria Isabel Pividori, 2021, Sensors 2021, 21(5), 1749; https://doi.org/10.3390/s21051749

Immunomagnetic separation of Salmonella with tailored magnetic micro and nanocarriers. In Salmonella Methods and Protocols (third edition), ‘Methods in Molecular Biology’ by Humana Press, 2021, 2182:51-65. doi: 10.1007/978-1-0716-0791-6_7. 

2020

Electrochemical immunosensing of nanovesicles as biomarkers for breast cancer. Silio Lima Moura, Carme García Martín, Mercè Martí, María Isabel Pividori.  Biosensors and Bioelectronics 150, 111882. https://doi.org/10.1016/j.bios.2019.111882. 

Multiplex detection and characterization of breast cancer exosomes by magneto-actuated immunoassay. Silio Lima Moura, Carme García Martín, Mercè Martí, María Isabel Pividori. Talanta, 211, 120657. https://doi.org/10.1016/j.talanta.2019.120657.

Osteoblastic exosomes. A non-destructive quantitative approach of alkaline phosphatase to assess osteoconductive nanomaterials. Maria Alejandra Sanchez, Betiana Felice, Luciano Sappia, Silio Lima-Moura, Mercè Martí, María Isabel Pividori. Materials Science & Engineering C, 115, 110931. https://doi.org/10.1016/j.msec.2020.110931

Matrix effect in the isolation of breast cancer-derived nanovesicles by immunomagnetic separation and electrochemical immunosensing. A comparative study. Silio Lima Moura, Mercè Martí, María Isabel Pividori. Sensors 20(4), 965. 

Determination of Temporary Dye Basic Red 51 In Commercial Hair Dye, River Water And Wastewater From Hairdressing Salon Using Graphite-Epoxy Composite Electrode Modified With Magnetic Nanoparticles. GT Corrêa, JC de Souza, JP Silva, MI Pividori, MVB Zanoni. Microchemical Journal, 105485, in press. https://doi.org/10.1016/j.microc.2020.105485

Biotinylated phosphorus dendrimers as control line in nucleic acid lateral flow tests. Laura Vidal, Alejandra Ben Aissa, Jordi Salabert, Jose Juan Jara, Adelina Vallribera, María Isabel Pividori, Rosa María Sebastián. Biomacromolecules, 2020, 21, 3, 1315-1323. https://doi.org/10.1021/acs.biomac.0c00161. 

Synthesis and characterization of a new ceramic nanomaterial Sio₂/Npssm₂o₃/C-graphite for the development of electrochemical sensors. Rodrigo Vieira Blasques, …, Jean-Louis Marty, María Isabel Pividori Gurgo, Gilvanda Silva Nunes, Paulo Cesar Mendes Villis. Materials Chemistry and Physics, 243, 122255.  https://doi.org/10.1016/j.matchemphys.2019.122255k