Structure of biomolecules and Biorecognition. Antibodies. Part 2

Lesson 2

February 18 @ 10:00 – 11:00 CET

Structure of biomolecules and Biorecognition. Amino Acids, Peptides and Proteins. Antibodies. Enzymes. Nucleic Acids. Biorecognition: Enzyme/Substrate. Antigen/antibody. Hybridization. Other affinity interactions in nature. Strept(avidin), Protein A and G. Aptamers. Biomimetic recognition. The importance of water in biorecognition. Biological buffers.

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This lecture will be focused on the structure of antibodies. The immune response and the production of antibodies will be discussed with the immunological techniques.

Crossword

Instructions. Solve the crossword, do a screenshot and post it in the forum under the topic Top five challenge!

1. Generic name for a group of glycoproteins find in the serum or in the tissues fluids of all mammals

2. Family of proteins that can be found free in the serum, popular for the Y shape

3. The Fab has the property to bind the .....

4. Heavy chain of the IgG class

5. Polypeptide chain of lower molecular weight in the antibody

6. Type of domain that is in contact with the antibody

7. The most frequent class of immunoglobulin

8. Structure of the IgM

9. Enzyme that cleave the immunoglobulins

10. bond which hold the antibody chains together

11. Number of heavy constants domains in an IgG

12. Number of polypeptide chains in an IgG antibody

13. IgM, IgA, IgG, IgE, and IgD are the antibodies......

14. number of IgG subclasses

15. One of the isotopes for the light chains in immunoglobulins

16. Part of an antibody which provides flexibility

17. Fc, constant part of the antibodies

18. IgG1, IgG2, IgG3, IgG4 are IgG....

19. Type of globular domains that take parts of Fc

Congratulations! You solve the antibody crossword!

Self-assesment quiz (not evaluable)

4 votes, 4.3 avg

Note!! After timer finished countdowning, quiz will be submitted automatically

Antibody structure (self assesment)

Self Assessment Test. This test will help you to revise the separation methods of the proteins and DNA.

25 questions in 1 page and 30 min!

Hint 1. the response can be multiple or, in most of the instances, simple. You will notice by clicking twice if the question admit only one, or more responses.

1 / 25

Which of the following statements is TRUE of IgE?

IgE is abundant in saliva

IgE binds strongly to mast cells

IgE cannot bind to macrophages

IgE activates the complement cascade

IgE has an insignificant role in worm infestations

2 / 25

Name the heavy chain of immunoglobulin M

3 / 25

Which of the following is the main immunoglobulin in the gut and secretions (saliva, milk, tears) and is important in mucosal immunity?

IgA

IgD

IgE

IgG

IgM

4 / 25

Which of the following is the largest immunoglobulin?

IgA

IgE

IgG

IgM

5 / 25

Which of the following factors is NOT true about the immunoglobulin structure?

Each immunoglobulin has two small chain termed light chain and two big chains called heavy chains.

The stem of the molecule consists of the variable regions of both heavy and light chains containing the N-terminal half of the Fab

The variable region is responsible for the highly specific antigen-recognition function of an individual antibody molecule for its antigen.

Each IgG molecule is composed of two identical light chains and two identical heavy chains which form a Y-shaped structure

6 / 25

Name the heavy chain of immunoglobulin G

7 / 25

In the antigen-antibody interaction, IS FALSE that it is established by :

hydrogen bridges

van der Waals forces

hydrophobic interactions

covalent bonds

ionic interactions

8 / 25

Which region of the immunoglobulin molecule can bind antigen?

Fab

Hinge region

9 / 25

Pepsin cleaves what region of immunoglobulin?

variable heavy chain domain

variable light chain domain

Hinge region

antibody binding site

10 / 25

Antigen binding sites of an immunoglobulin are located in

light chain alone

heavy chain alone

Fc region of the antibody

Fab regions of the antibody

11 / 25

Match the following descriptions with their term:

Has a crystallizable fragment (Fc) and Fab region

Light chain

Heavy chain

Fab region

Fc region

Hinge region

12 / 25

A fragment antigen binding (Fab) fragment:

Is produced by pepsin treatment

Is produced by separation of heavy and light chains

Binds antigen

Lacks light chains

Has no interchain disulfide bonds

13 / 25

Which of the following is a function of B lymphocytes?

Ingest large particles and cells by phagocytes

Produce and secrete antibodies

Interact with infected host cells through receptors on T-cell surface

Interact with macrophages and secrete cytokines

14 / 25

The most abundant immunoglobulin is

IgA

IgE

IgG

IgM

15 / 25

Name the heavy chain of immunoglobulin A

16 / 25

The correct sequence for the primary immune response is:
1) Membrane-bound antibodies recognize its targeted antigens
2) Secreted antibodies recognize its targeted antigens
3) Plasma cells secrete antibodies
4) B-lymphocytes proliferate and differentiate

2,4,3,1

4,3,1,2

4,1,3,2

1,4,3,2

17 / 25

IgG consists of

A light chain and two heavy chains joined by disulfide bond

Two light chains and a heavy chain joined by disulfide bond

Two light chains and two heavy chains joined by disulfide bond

18 / 25

Antigenic determinants bind to which portions of an antibody?

variable regions

constant regions

only light chains

only heavy chains

the effector region

19 / 25

Which of the following statements is NOT TRUE of affinity?

A measure of the strength of the binding of antigen to antibody

The equilibrium constant of the Ag/Ab complex

Avidity

Related to the free energy change of the Ag/Ab interaction

Related to specificity

20 / 25

Which of the following statements is TRUE about an epitope?

An epitope is the area on an antigen that contacts antibody

An epitope is the area on an antibody that contacts antigen

An epitope requires both antigen-binding arms of the antibody molecule for its recognition

An epitope is usually composed of a linear sequence of amino acids

An epitope is usually associated with a concave region of the antigen

21 / 25

J chain is present in

IgA

IgM

IgE

IgA and IgM

22 / 25

Which of the following is NOT true?

The term epitope is not synonymous with antigen

A viral protein may contain a large number of epitopes that are capable of interacting with many different specific antibodies

Antibodies can recognize both continuous and discontinuous epitopes

The term antigen is synonymous with immunogen

23 / 25

Gamma globulin serum fraction contains predominantly which immunoglobulin?

IgA

IgD

IgE

IgG

IgM

24 / 25

Cleavage of an IgG molecule by the protease, papain, produces:

an antigen-binding site and two constant regions

two heavy chain-light chain dimers

an inactive mixture of oligopeptides

two Fab fragments and one Fc fragment

an antibody without its N-linked oligosaccharide

25 / 25

Which of the following statement about the immunoglobulins is TRUE?

It is a very homogeneous family of serum proteins

The polypeptide chains of the immunoglobulins are linked by hydrogen bridges

IgG-class of immunoglobulin is bivalent (it has two antigen-binding sites per molecule)

The heavy chains of immunoglobulins have constant N-terminal domains

Immunoglobulin light chains have variable C-terminal domains

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Structure of biomolecules and Biorecognition. Antibodies. Part 2

Lesson 2

Podcast with the instruction for today

This lecture will be focused on the structure of antibodies. The immune response and the production of antibodies will be discussed with the immunological techniques.

Crossword

Congratulations! You solve the antibody crossword!

Self-assesment quiz (not evaluable)

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